Analysis status
Ready for genomic validation
The genome service resolves the locus, confirms the PAM, reports the cut site, and unlocks nearby guide search plus FASTA homology arms.
Inserted gRNA result
ReadyNo guide sequence detected
Weighted score-
Length0 nt
GC content0%
PAMAnalyze to find
flyCRISPR specificity-
DRSC/Housden-
Platinum criteria-
CRISPRDB ensemble-
Experimental evidenceNot found
Specificity-
- Enter a 20-nt gRNA sequence to begin.
Nearby guide search
Not resolvedAsk for a better gRNA near the binding site
Choose a resolved genomic site, then search nearby.
Genome match lookup
Genome check pendingFind where the 20-bp gRNA maps
Full-genome coordinatesNot checked
Genome hits0
Press Analyze to search the installed Dmel genome index.
FlyGuide searches the indexed dm6 genome on both strands, returns chromosome coordinates, and confirms adjacent PAMs. Additional exact matches are reported as specificity warnings.
Enter a 20-bp gRNA and select Analyze. Optional gene or coordinate context can help choose among multiple matches.
Choose intended genomic siteNot resolved
Awaiting input
Enter a 20-bp gRNA
-Weighted score-Exact off-targets
Not searched
Use nearby search after resolving the binding site
-Weighted score-Predicted OT ≤3mm
Region
Analyze to resolve the locusTarget locus map
No genomic region resolved
canonical-transcript exon genomic interval zero predicted sites within 3 mismatches predicted off-target warning
Molecular view
Entered gRNAPost-cleavage Cas9 position
chromosome 5'chromosome 3'
PAM-distal sequencePAM-proximal sequence
opposite strandCas9 retained side
double-strand break
PAM Analyze to find
Cas9
+ strand guideCas9 remains bound on the right side of the break, toward the PAM-proximal end of the guide on the chromosome 3' side.
Candidate ranking
Analyze to resolve locusEntered guide and nearby alternatives
GuideSequenceScoreOTPAMStatus
Entered---Analyze to findAwaiting input
Use “Find better nearby guides” to fill this table with genome-derived alternatives around the selected locus.